Spatial-Spectral Fluorescence Imaging:
A three-dimensional imaging system incorporating multiplexed holographic gratings is able to visualize fluorescence tissue structures. Holographic gratings formed in volume recording materials such as PQ-PMMA photopolymer have narrowband angular and spectral transmittance filtering properties which enable obtaining spatial-spectral information within an object. The imaging system’s ability is demonstrated to obtain multiple depth-resolved fluorescence images.
Figure on the left shows the experimental system setup, and figure on the right shows two depth-resolved images of a mouse colon.