光學顯微影像系統實驗室的主持人為 駱遠副教授，其專長領域包含3D光學顯微系統 ( 3D optical microscopic system )、超快內視鏡技術 ( High-speed optical endoscopy )、多焦聚光學全相鏡頭 ( Multi-focal volume holographic lenses )、非侵入式光學影像與設計 ( Non-invasive optical microscopy and design )、繞射光學造影 ( Diffraction optics )。
Multiplane Holographic Non-scanning microscopy:
Phase-preserved macroscopic visible-light carpet cloaking beyond two dimensions:
Nano-SiO2 in PQ-PMMA for Holographic Filters in Imaging:
Holographic filters in imaging/data storage/communications are required to have high Bragg selectivity, namely narrow angular and spectral bandwidth, to obtain spatial-spectral information within a three-dimensional object. The holographic filters with optimized ratio of nano-SiO2 in PQ-PMMA can significantly improve the performance of Bragg selectivity and diffraction efficiency by 53% and 16%, respectively.
Images at two depths within a grapefruit obtained using two multiplexed holographic nano-SiO2 PQ-PPMA filters.
Wavelength-Coded Holographic Microscopy:
A wavelength-coded multifocal microscope incorporates multiplexed and wavelength-coded holographic gratings to generate wavelength-selective multifocal planes. The focal planes are longitudinally spaced on the object plane, and each focal plane is probed by a designated wavelength. The recording of the multiplexed gratings takes place at a single wavelength by utilizing the Bragg degeneracy property; thus the maximum sensitive wavelength of blue 488 nm is used for recording, but the device is operated at a broad wavelength band of interest, all the way to red 633 nm. Figure on the left shows Two depth-resolved images of an onion obtained with wavelength-coded multifocal microscopy using both blue and red LEDs for illumination. Figure in the middle shows One of the two depth-resolved images obtained with wavelength-coded multifocal microscopy when the blue LED is on and red one is off.
Figure on the right shows One of the two depth-resolved images obtained with wavelength-coded multifocal microscopy when the red LED is on and blue one is off.
Spatial-Spectral Fluorescence Imaging:
A three-dimensional imaging system incorporating multiplexed holographic gratings is able to visualize fluorescence tissue structures. Holographic gratings formed in volume recording materials such as PQ-PMMA photopolymer have narrowband angular and spectral transmittance filtering properties which enable obtaining spatial-spectral information within an object. The imaging system’s ability is demonstrated to obtain multiple depth-resolved fluorescence images.
Figure on the left shows the experimental system setup, and figure on the right shows two depth-resolved images of a mouse colon.